منابع مشابه
On the Equivalence of Fcs and Frap: Simultaneous Lipid Membrane Measurements by Imaging Fcs
Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery after Photobleaching (FRAP) are probably the most widely used methods to determine diffusion coefficients in biological samples. However, it has already been noted in the past that the two methods do not necessarily provide the same results. This has typically been explained by the different spatial range over which the two m...
متن کاملOn the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements.
Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determine diffusion coefficients. However, they often do not yield the same results. With the advent of camera-based imaging FCS, which measures the diffusion coefficient in each pixel of an image, and proper bleaching corrections, it is now possible to measure the diffus...
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Improved techniques for measuring horizontal and vertical wind components and state variables on research aircraft are presented. They include a filtering method for correcting ground speed and position Inertial Navigation System data with Global Positioning System data, use of moist-air thermodynamic properties in the true airspeed calculation, postflight calculation of the aircraft vertical v...
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Biofilms are recognised as an important contributor to bacterial resistance towards traditional antimicrobial treatments. Assessment of biofilm formation currently relies on a 96 well microtitre plate assay, which usually involves the colourimetric detection of stain (typically crystal violet) removed from previously stained biofilm. The amount of crystal violet released is then used as a quant...
متن کاملStraightforward FRAP for quantitative diffusion measurements with a laser scanning microscope.
Confocal or multi-photon laser scanning microscopes are convenient tools to perform FRAP diffusion measurements. Despite its popularity, accurate FRAP remains often challenging since current methods are either limited to relatively large bleach regions or can be complicated for non-specialists. In order to bring reliable quantitative FRAP measurements to the broad community of laser scanning mi...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2020.03.017